A pioneering technique that a Universitat Rovira i Virgili, the Ohio State University and University of Texas researcher team has helped to develop enables this chronic disease to be identified more quickly and precisely

A research team from the Universitat Rovira i Virgili, The Ohio State University and the University of Texas has developed an innovative method that allows fibromyalgia to be reliably diagnosed from a blood sample. Identifying this disease nowadays is highly complex, especially because the symptoms are similar to those of other rheumatic pathologies and persistent Covid. This new study represents an important step forward in the accurate diagnosis and personalisation of treatment for affected individuals, and at the same time opens new avenues for research into this pathology and clinical care. The results of the study have been published in the journal Biomedicines.

Fibromyalgia is a chronic disease that affects 6% of the population worldwide and is characterized by widespread muscle pain and fatigue. The variety of symptoms can easily be confused with other disorders such as rheumatoid arthritis, systemic lupus erythematosus, osteoarthritis or chronic low back pain. This means that reaching a diagnosis is not easy or quick and, therefore, ends up having an impact on the quality of life and mental health of the people who suffer from it.

To carry out the study, the research team collected blood samples from three different groups: people diagnosed with fibromyalgia, people with similar rheumatic diseases and people without any of these pathologies, who served as a control group. A combination of techniques were used to isolate and analyse specific chemical signals in the blood that could help differentiate fibromyalgia from other diseases with similar effects on health, with the aim of opening a more accurate and faster diagnostic pathway.

The blood samples underwent a process called filtration, which allows the focus to be put on a very specific part of the blood that contains small molecules, known as the low molecular weight fraction. These molecules can include a variety of substances, for example amino acids, which act as the building blocks of proteins.

To analyse these tiny molecules, the researchers used the technique of Raman spectroscopy, which consists of “illuminating” the samples with a special laser light and studying how they react. This technique makes it possible to observe which molecules are present and in what quantities, based on the way light interacts with them. To further refine this process, gold nanoparticles were used, which increase the signal they receive from the small molecules and make the results clearer and easier to interpret.

With the help of a statistical method, the samples were classified into categories on the basis of their chemical characteristics so that the differences between groups could be determined. The results highlighted that some patterns in small molecules, such as amino acids, could serve as “chemical signatures” to distinguish fibromyalgia from other diseases.

“This tool is fast, accurate and non-invasive, and can easily be integrated into the clinical environment to improve the quality of life of patients with fibromyalgia”, says Sílvia de Lamo, a researcher from the URV’s Chemical Engineering Department, who played a part in the research during a stay at the Ohio State University, where she collaborates with two research groups specialising in vibrational spectroscopy as a method for diagnosing diseases. The tool is still in the validation phase.This research is funded by the National Institute of Health of the United States.

Reference: Bao, H.; Hackshaw, K.V.; Castellvi, S.d.L.; Wu, Y.; Gonzalez, C.M.; Nuguri, S.M.; Yao, S.; Goetzman, C.M.; Schultz, Z.D.; Yu, L.; et al. Early Diagnosis of Fibromyalgia Using Surface-Enhanced Raman Spectroscopy Combined with Chemometrics. Biomedicines 2024, 12, 133. https://doi.org/10.3390/biomedicines12010133